T4 dna ligase neb m0202s
WebLigations were performed using T4 DNA ligase ( NEB, cat. M0202S) and a 2 h incubation at RT followed by overnight at 16 °C. The ligated products were purified using December 1, 2024 Julie K. Fierle, Johan Abram-Saliba, Vasileios Atsaves, Matteo Brioschi, Mariastella de Tiani, Patrick Reichenbach, Melita Irving, George Coukos, Steven M. Dunn Webwww.neb.com [email protected] New England Biolabs Certificate of Analysis M0202L / Lot: 10183117 Page 1 of 3 Product Name: T4 DNA Ligase Catalog Number: M0202L Concentration: 400,000 U/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ... PS-M0202S/L v1.0 T4 DNA Ligase Component List
T4 dna ligase neb m0202s
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WebT4 DNA Ligase M0202S/L 25°C (4–37°C) Y (65°C) ATP Ligation of nicks in dsDNA and joining dsDNA fragments with complementary overhangs > 2 bases in length. Routine cloning. T4 DNA Ligase can ligate other substrates with reduced efficiency, including: nicks containing mismatches and 1 or 2 nt gaps; dsDNA fragments with blunt ends and single … Web1X T4 DNA Ligase Reaction Buffer 組成: ・50 mM Tris-HCl ・10 mM MgCl2 ・1 mM ATP ・10 mM Dithiothreitol pH 7.5 @ 25°C 酵素溶液の組成: 50 mM KCl、10 mM Tris-HCl …
WebNov 18, 2024 · The RT Adaptor (RTA) was ligated to the 3’ end of Poly(A)+ RNA by T4 DNA ligase (NEB M0202S) and then reverse transcribed by SuperScript III Reverse Transcriptase (ThermoFisher 12574018). The RNA was purified by 1.8x RNAClean XP beads (72 µL) (Beckman Coulter A63987) and then the RNA Adaptor (RMX) was ligated … Web1 uL Vector DNA 3 uL Insert DNA 1 uL 10x Ligase Buffer 1 uL T4 DNA Ligase 4 uL H 2 0 (to bring total volume to 10 uL) Tip: Try different vector to insert ratios to optimize ligation reaction. Tip: Do negative controls: vector only, insert only, no ligase. b. Incubate at 16 o C for 2 hours, or at 4 o C overnight (follow the manufacturer's ...
WebEnter the email address you signed up with and we'll email you a reset link. WebT4 DNA Ligase from multiple suppliers was tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3'overhang or 5' overhang containing oligonucleotides. The percent degradation by …
WebDNA fragments (vector, insert) • T4 DNA ligase (5 U/µl; Invitrogen, Cat. No. 15224-041) • 5× ligase reaction buffer (from 20 µl aliquots) Procedure 1. Add 4 µl 5× ligase reaction buffer, 4 µl annealed oligonucleotides, 50 ng Bbs I/ Mlu I restricted vector DNA, and 1–2 µl ligase (5 U/µl) into a clean tube. 2.
WebT4 DNA Ligase, Article Number: M0202 Sign In (0) Products & Applications. back Cloning / Synthetic Biology ... M0202S T4 DNA Ligase Packaging Unit: 20.000 units € 72,00 -+ … counselling services in cheshireWeb丁香通为您找到10条dna杂交封闭试剂信息，包括dna杂交封闭试剂报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购dna杂交封闭试剂,上丁香通移动站。 counselling services in buryWebProduct Name: T4 DNA Ligase Catalog #: M0202S/L Concentration: 400,000 units/ml Unit Definition: One unit is defined as the amount of enzyme required to give 50% ligation of 6 µg of Lambda-HindIII DNA in 30 minutes at 16°C in a total reaction volume of 20 µl. counselling services in greater manchesterWeb1 μL NEB T4 DNA ligase add ddH 2 O to bring to 20 μL final volume Incubate at 16°C for 4-20 hours. Transform 2 μL of ligation mix into 25 μL competent DH5 alpha cells, following manufacturer’s protocol. Plate on LB agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog). counselling services in darwinWebLigation Protocol with T4 DNA Ligase (M0202) NEB Applications & Products Applications Cloning & Synthetic Biology DNA Amplification, PCR and qPCR Genome Editing RNA … counselling services in chelmsfordWebT4 DNA Ligase will ligate these substrates: dsDNA Nicked DNA/RNA Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl … brelshaza with pupilsWebFor blunt ends, use 1 µl of T4 DNA Ligase in a 20 µl reaction for 2 hours or 1 µl high concentration T4 DNA Ligase for 10 minutes. Alternatively, NEB's Quick Ligation Kit (#M2200S) [30 reactions] or (#M2200L) [150 reactions]) is uniquely formulated to ligate both blunt and cohesive (sticky) ends in 5 minutes at room temperature. References counselling services in gaborone