Snarf-1 fluorescence
Web3. pH-dependent spectra of water-soluble SNARF Figure S3: pH-dependent fluorescence spectra of water-soluble SNARF. Fluorescence emission spectra of water-soluble SNARF in buffer solutions of different pH upon excitation at 543 nm. All measurements were made in pH-adjusted buffers of the indicated pH values Web25 May 2024 · Intensity images of the SNARF-1 fluorescence were collected using two-photon microscopy (as described above) for both experimental dishes (cells treated with pH-adjusted HEPES-containing DMEM media, no nigericin) and calibration dishes (cells treated with nigericin buffer solutions). The laser was tuned to 900 nm (average power: 0.3 mW) …
Snarf-1 fluorescence
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Weband co‑workers in 1982,1 is the most widely used fluorescent indicator for intracellular pH. Several characteristics contribute to this widespread utility: The pK a of 7.0 is ideally matched to the normal range of cytoplasmic pH (~6.8–7.4). The fluorescence excitation profile is pH‑dependent (Figure 1), allowing the implemen‑ Web1 (SNARF-1) loaded K562 cells. Materials and methods A device for quantitative fluorescence microscopy A schematic representation of the device is shown in Fig. 1. A fiber (the refractive indices (n) of core and cladding being 1.460 and 1.404, re- spectively; N.A. 0.40, Cunz, Frankfurt/M, Ger-
Web1 Dec 2002 · In this paper, we describe two methods to measure pH by fluorescence spectroscopy and a way to extend the measurement to living cells. We use the fluorescent probe, C-SNARF-1, which exhibits a spectral shift of 45 nm upon proton binding. The two-wavelength-ratio method is applied to the determination of an unknown pH in aqueous … Weband stability of cellular fluorescence. SNARF-1 had a practical and stable ex- citation wavelength of 488 nm rather than UV, it offered the possibility of ratio- metric measurements on the basis of a real emission shift, and had superior res- olution for the pH range 7-8. With SNARF-1 we found that keratinocytes
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Web12 Jun 2015 · SNARF was excited at 543 nm, and fluorescence recorded between 560 and 750 nm. For calibration curves, the ratio of intensities of low-wavelength maximum (at 580 nm) and the high-wavelength maximum (at 630 nm for soluble SNARF, and 650 nm for SNARF-DOPE) was calculated.
Web18 Jul 2024 · Seminaphtho r hoda f lours-1 (SNARF-1) shows varying emission intensities at wavelengths of around 580 nm and 640 nm, depending on the pH when excited by a 488-nm laser (Whitaker et al. 1991). The SNARF-1 can pass through the PM of tobacco BY-2 protoplast and cell wall and the PM of Arabidopsis suspension cells and monitor cytosolic … did the apostle paul keep the sabbathWeb24 Aug 2016 · The fluorescent platform of carboxy-SNARF-1 is asymmetric, as it is built in half by naphthofluorescein and in half by the tetramethylrhodamine probe. Because both of these fluorescent probes demonstrate different emission properties, carboxy-SNARF-1 exhibits two independent emission bands. did the apostle paul perform any miraclesWebSNARF-1 in solu- tion was used to calibrate pH e measurements. Separate experiments were used to measure pH i or pH e because of the difficulty of balancing both signals in a single experiment... did the apostle paul see heavenWebUTX-40, SNARF-OAM or SNARF-OAc (10 μM) in the assay buffer (10 mM HEPES buffer (pH 7.5)) at 20 ± 1 °C was mixed with esterase (final concentration: 0.5 U/ml), respectively. The fluorescence spectra (excitation wavelength: λex = 534 nm) and UV–vis spectra were monitored for 180 min. The did the apostle paul speak aramaicWebCells labeled with our SNARF-1 carboxylic acid, acetate, succinimidyl ester have orange-red fluorescence that can be easily distinguished from that of cells loaded with green-fluorescent tracers such as CFDA SE. Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators › did the apostle paul speak greekWebThe pH-sensitive fluorescent dyes, fluorescein and C-SNARF 1, have been used to generate pH maps of microfluidic devices with a time-gated camera and a time-and-space-correlated single photon counting (TSCSPC) detector, respectively. Using time-gated detection and fluorescein, the fluorescence lifetime images allow for direct reading of the pH. did the apostle paul really existWeb1 Jan 2012 · Alternatively, the fluorescence of SNARF-1 free acid (100–200 μM) in solution can be imaged through the microscope optics as pH is varied. After background subtraction, the >620-nm image channel is divided by the 585-nm channel on a pixel-by-pixel basis. Using thresholding to eliminate low pixel values of the extracellular space, a standard ... did the apostle paul struggle with sin