Overexpression or knockdown
WebDec 25, 2024 · Recently, we knockdown a non-coding RNA by siRNA in 293T cell line. This non-coding RNA was proved to be highly expressed in 293T cell lines, and the exact … Web3. Under the basal conditions, transient overexpression of CPR protein in WB-F344 cells caused a 90% increase in the CPR activity, which was associated with a 100% increase in …
Overexpression or knockdown
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WebStable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected plasmid DNA or reporter gene constructs into … WebThe knockdown had no effect on target gene expression, whereas overexpression had a minor effect in the same cell line and reduced target gene expression. Any explanation or …
Organoids of small intestine, colon and stomach were established from freshly isolated glands from each organ. Incubation in PBS (pH 7.4) containing 2 mM EDTA (for small intestine) or 5 mM EDTA (for stomach and colon) was used for the isolation of glands. The culture condition of mouse small … See more A dsRed cassette from dsRed-express2 (Clontech) was introduced into the BglII site of MSCV-puro (Clontech) with a loxP site on each side. Three consecutive … See more The knockdown vector was generated based on the MSCV FLIPi P2G_Thy1.1 p53 plasmid (Addgene, 19748)13. First, we changed eGFP into dsRed from dsRed … See more Sequence encoding mouse Math1 (Atoh1) and a fragment encoding amino acids 13–74 of human MAML1 (dominant negative form of MAML1) were cloned into … See more Five DNA sequences (Supplementary Table 1) were annealed and ligated into linearized pcDNA6.2-GW/EmGFP-miR (Invitrogen). The whole emGFP-miRcassette … See more WebCyclin E overexpression resulted in resistance to trastuzumab both in vitro and in vivo. Inhibition of cyclin E activity in cyclin E-amplified trastuzumab resistant clones, either by knockdown of cyclin E expression or treatment with cyclin-dependent kinase 2 (CDK2) inhibitors, led to a dramatic decrease in proliferation and enhanced apoptosis.
WebHowever, transient or stable knockdown of caspase-8 or overexpression of CrmA inhibited apoptosis. Cell death was not inhibited by preventing death receptor-ligand interactions, by overexpression of c-FLIP or by knockdown of RIPK1. Glucose deprivation induced apoptosis in the human tumor cell line HeLa, which was prevented by knockdown of ... WebCells were infected with LINC00461 overexpression, miR-411-5p overexpression, or corresponding knockdown, lentiviral particles. After 2–3 days, levels of green fluorescent …
WebAdditionally, knockdown of KIF4A reduced migration and invasion of UBC cells whereas overexpression of KIF4A exhibited opposite effects, along with altered protein level in …
WebApr 14, 2024 · Initially, the co-immunoprecipitation approach was employed to confirm the relationship between USP5 and PARP1. Reciprocal IP results showed that USP5 … the 4 e\u0027sWebMoreover, the CD133+ percentage of cells with PDK4 overexpression was significantly greater than that of the control cells, while knockdown of PDK4 inhibited CD133+ … the 4 es of marketingWebDec 25, 2024 · Abnormal accumulation of alpha synuclein (α-Syn) in sporadic and familial Parkinson’s disease (PD) may be a key step in its pathogenesis. In this study, the expression matrix of the GSE95427 dataset after α-Syn overexpression in human glioma cell line H4 was obtained from the GEO database. We used the Gene Set … the 4 falls of buffaloWebApr 12, 2024 · To address this question, we first evaluated the effect of NCoR1 overexpression in MEF cells. As expected, NCoR1 overexpression decreased the expression of PPAR gene targets (Suppl. Figure 1 A and B). Conversely, its knockdown increased the oxygen consumption rate (Suppl. Figure 1 C and D). the 4 factors influencing corrosionWebApr 12, 2024 · The level of miR-340-5p was downregulated by circSmox overexpression but upregulated by circSmox knockdown in PC12 cells (Figure 3G). Then the knockdown efficiency of miR-340-5p inhibitor was validated by qRT-PCR analysis (Figure 3H ), and we proved that miR-340-5p inhibitor overtly reduced circSmox knockdown-triggered elevation … the 4 fat soluble vitaminsWebApr 9, 2024 · Moreover, TCF7 knockdown suppresses cervical tumor growth. Lnc34a shows high levels in colon CSCs, causes an asymmetric division of CSCs, and improves colon cancer proliferation by sponging miR-34a . LNCBRM overexpression enhances colon cancer cell proliferation and invasion by sponging miR-204-3p, which is reversed by LNCBRM … the 4 evangelistsWebThe cell proliferation after Gαi2 knockdown or overexpression was evaluated by Cell-Light™ EdU Apollo®488 In Vitro Imaging Kit (RiboBio) according to the manufacturer's instructions. Briefly, cells were labelled with 5-ethynyl-2′-deoxyuridine (Edu, RiboBio) at a final concentration of 50 μM for 2 h. the 4 fires in ordina