Fpn antibody
WebFeb 12, 2014 · The eluted samples from IP of Fpn were separated as described and assayed for Fpn (ab85370) or ubiquitin (FK2 antibody; Enzo Life Sciences, BML-PW8810, Farmingdale, NY). Fpn and FK2 primary antibodies were detected using the Clean-Blot IP Detection Reagent (HRP) (Thermo Scientific, Pittsburgh, PA) as per the manufacturer's … WebNov 8, 2024 · The protein samples with loading buffer were incubated at 37°C for 15 minutes for antibodies of TfR1, FPN, DMT1, and band 3 or at 95°C for 5 minutes for the other antibodies. After mixing and briefly centrifuging, the samples were separated on 4% to 12% Bis-Tris gels (Thermo Fisher Scientific), and then transferred to nitrocellulose …
Fpn antibody
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WebAntibody: Immunogen: DMT1 fusion protein Ag14314: Full Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2: Calculated molecular weight: 568 aa, 62 kDa: Observed molecular …
WebWestern analysis results for two anti-human FPN antibodies (38C8 and 31A5), along with control anti-V5 antibody are shown in Figure 2A. FPN comprises 571 amino acids and … WebOct 31, 2006 · That the antibody detected endogenous Fpn was shown by the loss of both bands when antibody was pretreated with the recombinant Fpn-specific peptide (Figure 4A) as well as the loss of the specific protein band upon incubation of cells with hepcidin, the predicted consequence being the internalization and degradation of Fpn (data not shown ...
WebMay 29, 2024 · Images of FPN-GFP showed a similar pattern of fluorescence as the images obtained with an anti-FPN antibody, suggesting that the GFP signal represented subcellular FPN … WebAug 26, 2024 · The cell lysates were immunoprecipitated using an anti-FPN antibody and immunoblotted using the FK2 anti-polyubiquitin antibody. The red asterisk labels the polyubiquitinated FPN (C). (D) Quantification of FPN ubiquitination (n = 4 per group). (E) Schematic diagram depicting the main steps in screening for potential E3 ligase genes.
WebJun 6, 2012 · We thank Drs. Sandra Ross and Tara Arvedson (Amgen) for anti-Fpn antibodies and many valuable discussions. This work was supported by National Institutes of Health (NIH) grant R01 DK 082717 (to E.N.). Drs. Nemeth and Ganz are officers and shareholders of Intrinsic LifeSciences, LLC, a biotech company developing iron-related …
WebSep 28, 2024 · The pcDNA3.1 (+)-FPN, and empty plasmids were transiently transfected into HEK-293T cells by using Lipofectamine 2000 (Thermofisher), and after 48 h the transfected cells were collected and lysed in RIPA buffer supplemented with protease inhibitors (Sigma-Aldrich) for evaluating the activity and specificity of anti-FPN … tr weasel\u0027sWebJun 6, 2012 · (A) 293 T-REx/Fpn cells and JAK2 null γ2A/Fpn cells were induced to express Fpn, treated with control medium or 1 μM Hepc for 3 hr. Live cells were stained with Fpn antibody H1. Loss of cell-surface Fpn was quantified in 293 T-REx/Fpn cells and γ2A/Fpn cells (B) over time after treatment with 250 nM Hepc and (C) over a dose range of Hepc. trwebocr gpuWebMar 20, 2024 · Cells were plated in 8-chamber slides (BD Falcon, Big Flats, NY, USA) and incubated with anti-human FPN antibody 47 or anti-human TFR1 antibody (Invitrogen, cat# 13-6890). Rodamine-Red (Jackson ... trweb ocrWebView application images and datasheets for 227 anti Fpn1 Antibody antibodies from 21 leading antibody suppliers, plus reviews and the top related antibodies. Toggle … tr weathercock\u0027sWebAnti-Mouse Metal Transporter Protein1/Ferroportin (MTP1/IREG1/Fpn) IgG aff pure General Information A novel iron-regulated gene that is homologous to the DMAT1, termed Metal … tr weaponsWebAntibody: Immunogen: SLC40A1/FPN1 fusion protein Ag24273: Full Name: solute carrier family 40 (iron-regulated transporter), member 1: … trwebocr apiWebFeb 22, 2024 · Fpn antibody (G-16) was from Abcam and also from Santa Cruz Biotechnology. IRP1 and actin antibodies were obtained from Santa Cruz Biotechnology; IRP2 antibody from Alpha Diagnostic International. FBXL5 and FLAG antibodies were from Sigma-Aldrich. Antibodies for HIF-1α and HIF-2α were from Novus Biologicals and … trwebocr使用