2024 Bulk rna-seq macrophages adipose tissue

Bulk rna-seq macrophages adipose tissue

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August undefined, 2024

WebJul 15, 2024 · The results of RNA-seq and weighted gene co-expression network analysis (WGCNA) of chick subcutaneous and abdominal adipose tissues showed that the function of chick subcutaneous tissue was related to immunoreaction, and macrophage could be the major immune infiltration cell type in chicken subcutaneous adipose tissue, which … WebIn addition, we also performed bulk RNA and exome sequencing on the aforementioned ACP tumors to gain insight into their basic properties. The transcriptional signatures and activating mutations of CTNNB1 exhibited by these tumor tissues were consistent with the typical genomic pattern of ACP (fig. S1, C and D, and table S2) (12, 36 ...

Advanced analysis and applications of single-cell transcriptome sequencing

WebApr 24, 2024 · We applied our method to 106 bulk RNA-seq subcutaneous adipose tissue samples collected from both lean and obese individuals, where 6 samples have both bulk RNA-seq and snRNA-seq data available ... WebTumor-associated macrophages (TAMs) are a highly plastic stromal cell type that support cancer progression. Using single-cell RNA sequencing of TAMs from a spontaneous … the cw bakersfield

Frontiers Integrated Multiomic Analysis Reveals the …

WebMay 6, 2024 · Bone marrow adipose tissue reflects the accrual of adipocytes embedded within a complex extracellular matrix along with … WebThe increased number of adipose tissue macrophages (ATMs) in obese adipose tissue is in part due to recruitment of peripheral cells ( 5 – 7 ), but local proliferation also plays a … the cw app 90210

Comparative bulk and single-cell transcriptome analyses revealed ...

Vaccines Free Full-Text Integration of Bulk and Single-Cell RNA-Seq ...

WebJan 28, 2024 · To identify early changes in MS brain tissues, we here analyzed the transcriptional profile of NAWM tissues without apparent macrophage (either microglia … WebApr 12, 2024 · Aim Obesity is linked to cardiometabolic diseases, however non-obese individuals are also at risk for type 2 diabetes (T2D) and cardiovascular disease (CVD). White adipose tissue (WAT) is known to play a role in both T2D and CVD, but the contribution of WAT inflammatory status especially in non-obese patients with … the cw app for samsung smart tvWebAug 4, 2016 · The core macrophage program initiated in pMacs is rapidly diversified as expression of transcriptional regulators becomes tissue-specific in early macrophages. This process appears essential for macrophage specification and maintenance, as inactivation of Id3 impairs the development of liver macrophages and results in selective … the cw batwoman cast

"WebMay 11, 2024 · Single-cell RNA-sequencing (scRNA-seq) recently clarified the roles of different ATM subpopulations in obesity progression (Burl et al., 2024; ... the designation of macrophages as “adipose tissue macrophages” is then also an oversimplification. Different macrophage populations and depot-specific macrophage subpopulations with … " - Bulk rna-seq macrophages adipose tissue

Bulk rna-seq macrophages adipose tissue

A human adipose tissue cell-type transcriptome atlas

WebJun 1, 2024 · Figure 1. Adipose tissue macrophage landscape in lean, obese, and after caloric restriction–induced weight loss. The major leukocyte type in the white adipose tissue (WAT) is the macrophage. Increasing evidence show that adipose tissue macrophages (ATMs) are highly heterogeneous, and their landscape drastically … WebThe scRNA-seq dataset (GSE131882) contained 23,980 single-cell transcriptomes from three control and three diabetic kidney samples. 20 The bulk RNA-seq dataset (GSE142025) consisted of 27 patients with DKD and eight normal cases. 21 The GSE51674 dataset, which included the microRNA (miRNA) profiles of six patients with DKD and four …

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WebAccumulation of adipose tissue macrophages in obesity has also been shown in human WAT, using a combination of histomorphometry and flow sorting 19,22. ... Calculation of pseudobulk datasets to estimate adipose innervation. Approximate bulk RNA-seq datasets (pseudobulk) were obtained for visceral sNuc-seq samples by summing the total … WebSep 19, 2024 · Background: The plasma membrane provides a highly dynamic barrier for cancer cells to interact with their surrounding microenvironment. Membrane tension, a pivotal physical property of the plasma membrane, has attracted widespread attention since it plays a role in the progression of various cancers. This study aimed to identify a …

WebNov 15, 2024 · In adipose tissues with CAC, clear pro-inflammatory transitions were discovered in adipose progenitors, macrophages and CD8 + T cells, with dramatically remodeled cell interactome among these... WebApr 8, 2024 · Secondly, we have only performed bulk RNA-seq for mRNAs in UCB cells. As we know, UCB cells consist of a heterogeneous population of cells. ... Fain JN (2006) Release of interleukins and other inflammatory cytokines by human adipose tissue is enhanced in obesity and primarily due to the nonfat cells. ... Bucala R (2010) …

WebApr 12, 2024 · Chronic non-healing wounds, a prevalent complication of diabetes, are associated with increased mortality in diabetic patients. Excessive accumulation of M1 macrophages in diabetic wounds promotes inflammation and results in dysregulated tissue repair. Adipose tissue macrophages (ATMs) derived from healthy lean donors have … WebOct 23, 2024 · S ingle N uclei A di p ocyte RNA -seq uencing (SNAP-seq) of subcutaneous adipose tissue defined a metabolically-active mature adipocyte subtype characterized by robust expression of genes involved in thermogenesis whose transcriptome was selectively responsive to IL10Rα deletion.

WebFeb 2, 2024 · A well-known hallmark of obesity is the dramatic increase in adipose tissue-resident macrophages and an associated change in the cytokine profile, which have …

WebJul 7, 2024 · The number of macrophages in adipose tissue positively correlates with the exacerbation of metabolic syndrome and the secretion of proinflammatory mediators … the cw bay areaWebOct 22, 2024 · Q8. How much data can I expect from an RNA-Seq analysis of a mammalian RNA sample ? A8. A transcription profile (relative gene expression) of a sample can typically be made from 50 million clusters of reads. For detection of splice variants or low-expressing genes, then up to 150 million clusters of reads may be desirable. References : 1. the cw accountWebMay 5, 2024 · This in silico approach mimics bulk RNA-seq for the cell populations, ... Interestingly, CX3CR1 was recently shown to mark a unique population of tissue-resident ductal macrophages and TAMs in the mouse mammary gland (Dawson et al, 2024), though it is not expressed by normal breast tissue macrophages. the cw and netflixWebJun 28, 2024 · Results: The ChIP-seq and transcriptome analysis of mouse adipose tissues demonstrated that a series of genes were activated by the histone modification of … the cw batwomanWebApr 13, 2024 · In this study, HPH-related single cell sequencing (scRNA-seq) and bulk RNA sequencing (RNA-seq) data were downloaded from the public database of Gene Expression Omnibus (GEO) for bioinformatics analysis in order to find out genes with important regulatory roles in the development of HPH. 523 key genes were identified … the cw batmanWebThrough integrated analyses of the bulk RNA transcriptome and single-nuclei adipocyte RNA sequencing in mouse, rat and human adipose tissues, we found that a series of genes that were activated by histone … the cw commercials 2012WebIntegrated analysis of single-cell RNA-seq and bulk RNA-seq unravels tumour heterogeneity plus M2-like tumour-associated macrophage infiltration and … the cw channel number dish